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Live-Cell Fluorescence Imaging Chip
CIC-6F
Six-channel microfluidic imaging chip can use with a 170 µm optical bottom for confocal fluorescence microscopy, live-cell observation, blood cells, and micro-volume samples operation.
Fluorescence Microscopy Chip
Bring Small Samples into an Imaging-Ready Microchannel
Live-Cell Fluorescence Imaging Chip is a six-channel microfluidic observation chip developed for fluorescence and high-magnification microscopy. Samples remain inside defined microchannels, allowing researchers to work with small volumes while keeping cells or blood cells in an imaging-friendly format.
Optical Bottom Designed for Confocal Microscopy
The chip uses a 170 µm optical bottom, corresponding to the standard 0.17 mm coverslip thickness commonly used for high-resolution microscopy. This design supports confocal fluorescence microscopy and other high-magnification imaging workflows.
Six Independent Channels for Efficient Experimental Design
Each chip contains six short channels, allowing up to six independent experiments or conditions on a single slide-format device. Standard pipettes can be used to introduce cell suspensions or blood samples, making the chip easy to integrate into routine laboratory workflows.
FEATURE
- Compatible with confocal fluorescence microscopy and high-magnification observation.
- 170 µm optical bottom designed around standard microscopy optics.
- Six independent microfluidic channels on one chip.
- Supports observation, image capture, and video recording of rare or micro-volume samples.
- Suitable for high-sensitivity immunofluorescence staining workflows.
- Supports live-cell and blood-cell observation and research.Use small sample volumes to help conserve cells and reagents.
- Use small sample volumes to help conserve cells and reagents.
- Samples are contained within microchannels rather than spread across a conventional flat slide.
- Compatible with standard pipettes for introducing cell suspensions or blood samples.
- Can be used for cell and microbial experiments within the microchannels.
APPLICATION
Confocal Fluorescence Microscopy
Designed for high-resolution fluorescence imaging where optical compatibility and controlled sample placement are important.
Immunofluorescence Studies
Suitable for high-sensitivity immunofluorescence staining with limited sample volumes.
Live-Cell and Blood-Cell Observation
Supports direct microscopic observation of live cells and blood cells, including image capture and video recording.
Micro-Volume and Precious Samples
The microchannel format helps reduce sample and reagent consumption, making it useful when cells or reagents are limited.
Microbial and Cell-Based Experiments
The channels can be used as a compact environment for cell or microorganism observation and other microscopy-centered experimental workflows.
SPECIFICATION
| Item | Specification |
|---|---|
| Outer dimensions | 75 × 25 × 0.85 mm |
| Number of channels | 6 |
| Channel dimensions | 2 × 15 mm |
| Optical bottom thickness | 170 µm (0.17 mm) |
| Inlet / outlet hole size | Ø 2 mm each |
| Material | Polymer / Glass |
| Color | Transparent |
| Microscopy compatibility | Confocal fluorescence microscopy and high-magnification microscopy |
| Sample loading | Compatible with standard pipettes |
| Package | 12 pcs / box |
DIRECTION FOR USE
A general imaging workflow is provided below. Sample preparation, staining, incubation, and imaging conditions should be selected according to the experimental protocol.
- Prepare the cell suspension, blood sample, microorganism sample, or stained specimen according to the study protocol.
- Using a standard pipette, introduce the sample through the inlet and allow the microchannel to fill without trapping bubbles.
- Confirm that the sample is positioned in the observation region of the selected channel.
- Place Live-Cell Fluorescence Imaging Chip on the microscope stage and selecting the appropriate objective and imaging mode.
- For fluorescence experiments, use excitation, emission, exposure, and acquisition settings established for the fluorophore and microscope system.6. Capture images or video as required by the experiment.
- Capture images or video as required by the experiment.
- Use the remaining channels for additional replicates, conditions, or samples as appropriate.
- After completion, handle and dispose of the used chip according to the laboratory's biological-safety procedure.
Note: Recommended loading volume and incubation conditions are protocol-dependent.
FAQ
| 1. Is Live-Cell Fluorescence Imaging Chip compatible with confocal fluorescence microscopy? Yes. Live-Cell Fluorescence Imaging Chip is specifically designed to support confocal fluorescence microscopy and high-magnification observation. |
| 2. What is the optical bottom thickness? The chip uses a 170 µm, or 0.17 mm, optical bottom designed around standard high-resolution microscopy optics. |
| 3. How many experiments can be run on one chip? Live-Cell Fluorescence Imaging Chip contains six independent microfluidic channels, allowing up to six separate experimental conditions or replicates on one chip. |
| 4. Can I load samples with a standard laboratory pipette? Yes. The inlet and outlet design allows cell suspensions or blood samples to be introduced using a standard pipette. |
| 5. What types of samples can be observed? The product is intended for cell and blood-cell observation and can also be used for microbial experiments in microchannels. It is particularly useful for rare or micro-volume samples. |
| 6. Is Live-Cell Fluorescence Imaging Chip suitable for immunofluorescence? Yes. The product is described as suitable for high-sensitivity immunofluorescence staining and fluorescence imaging. |
| 7. How does the microchannel format help when samples are limited? Because the observation takes place in a defined microchannel using a small sample volume, researchers can reduce the amount of cells and reagents required compared with workflows that use larger sample areas or volumes. |
| 7. Can Live-Cell Fluorescence Imaging Chip be used for video or dynamic observation? Yes. The product supports image capture and video recording. Time-dependent or live-cell imaging can be performed when the microscope, environmental conditions, and experimental protocol are appropriate. |
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